Acrylamide Polymerization

raymond raymond
Thu Sep 11 14:58:29 EST 1997


HELP!

I am trying to refine the polymerization step in preparing the gel 
matrix for DNA sequencing.

It would help a lot if some bionet newsgroupies could enlighten me on 
the chemistry of ammonium persulfate (APS) and TEMED effect, initiation 
and control of acrylamide polymerization.

References to primary papers or reveiw articles would be especially 
helpful.

Raymond Henderson
All Children's Hospital
Dept. Molecular Genetics
St. Petersburg, Florida

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