Tue Sep 23 12:24:13 EST 1997
I wonder if any of the Li-Cor users out there can give me some help.
I keep getting what looks like unspecific termination on my gels. It
particularly bad on the pGEM samples. I get 60-80 bases of nice
then a blob of dye across all four lanes, then a really weak signal which
finally dies out. I add DMSO to my gel mix and the PCR reaction, I've
increasing cycle number and elongation times, but it has had little
I'm using the 4200, and multiplex sequencing, although I see the same
using a single primer.
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