Li-Cor help

A. A.
Tue Sep 23 12:24:13 EST 1997


Hi, 

I wonder if any of the Li-Cor users out there can give me some help.

I keep getting what looks like unspecific termination on my gels.  It 
looks
particularly bad on the pGEM samples.  I get 60-80 bases of nice 
material, 
then a blob of dye across all four lanes, then a really weak signal which 
finally dies out.  I add DMSO to my gel mix and the PCR reaction, I've 
tried
increasing cycle number and elongation times, but it has had little 
effect.

I'm using the 4200, and multiplex sequencing, although I see the same 
effect 
using a single primer.
 
--
Ang Rosin



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