SSCP using a 373

Ricky Peterson ricky_peterson at smtpgw.musc.edu
Fri Apr 3 15:09:59 EST 1998


I am considering way in which to detect mutations on a large number of DNA
samples.  I am wondering first of all whether SSCP is the way to go or just to
sequence the gene directly.  If I do proceed with the SSCP I would be
performing this on a ABI 373 using GENESCAN.  Doing it this way I will run
into the gel cooling problem.  Perkin-Elmer has a temperature control upgrade
for the 377 but not the 373.  Any ideas would be appreciated.

Ricky Peterson




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