robert_vogrig at hc-sc.gc.ca
Sat Aug 29 08:17:57 EST 1998
To all sequencers,
I have tried the membrane comb (a couple of times) put out by The
Gel Company and am having some difficulty getting reads past 400 bp.
I am using a 377, big dye and the gel pack put out by Mandel. The
wtr is 36 cm, regular 2* run.
I would run the temperature on the plates so as to reach 51 C, put
the membrane comb on the top of a dry gel, apply the buffer and do a 10
min pre run after which I would start the run.
The other difficulty is the applying of the comb to the dry gel.
After applying the sample to the wells, I wait 10 min. I apply
compressed ultra pure gas to the surface of the gel to dry it out. I
still get some wells that compress and do not touch the gel.
Any help would be greatly appreciated.
Health Canada Federal Laboratories
1015 Arlington St. Rm H3380
Email Robert_Vogrig at hc-sc.gc.ca
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