Bind Silane and 48 well combs - the answers

hoolwerf at solair1.inter.nl.net hoolwerf at solair1.inter.NL.net
Tue Feb 3 04:14:33 EST 1998


Hello,

Phillip San Miguel <pmiguel at bilbo.bio.purdue.edu> wrote:

 PSM>     We use bind silane with Alf express gels.  I hate the stuff,

It's a poison. But thta's not the greatest risk on a laboratory. <g>

 PSM> but it's a necessary evil if you want to keep the sides of the
 PSM> wells from moving around.

True. But i know that other users do not use it with every run but e.g. o=
nly
once in every 5 runs or so. Seems to work.

 PSM> We have a plastic scraper to remove the acrylamide left at the top
 PSM> of the gel.  I don't worry much about scratching the top edge of an
 PSM> Alfx gel =AD=AD the detectors are near the bottom.

I don't care about the plates at all. Most of the gel can be removed very
easily, also with the scaper, and i have not seen any scratching on the p=
late
we use for more than a year now. With about two runs a week i think.

 PSM> Then we wash as normal, using a teflon safe scrub sponge to remove
 PSM> the residual acrylamide from the top of the gel.

I wash the plates under continuously running tap water. With plenty of wa=
ter
the last traces of the gel are removed very easily and do not stick to cl=
ean
parts of the plates.

 PSM> I think too much bind silane can cause problems other than getting
 PSM> the plates clean. A Pharmacia rep said that an "ion front" migrates
 PSM> in the gel from the bind silane.  He suggested that  fluctuations w=
e
 PSM> were seeing in our voltage during a run were caused by this.

We use the standard bind silane method and i have no problems with voltag=
e
fluctuations.


with regards, Jan Hoolwerf

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**
 J.D. Hoolwerf
 Neth. Inst. for Dairy Research
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 the Netherlands                    e=ADmail : hoolwerf at solair1.inter.nl.=
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