I am wondering if any labs have altered the ABI 377XL running parameters for their acrylamide gels to yield a read length of 650 -700 bases with a trim length of 450-550 bases in a 3 hr run. If so, I would like the information. I am currently running for 3.5 hrs to obtain these results on 4.5% premixed Sooner gels. Thanks, Mark Mucha