fluorescent primer extention?

Moez TORKI Moez.TORKI at ujf-grenoble.fr
Fri Feb 20 13:09:44 EST 1998

<bigger><bigger>Dear Web molbiologists,

May be am I asking a stupid question, but has anyone tried a primer
extention experiment with an fluorescent primer (or ddNTP)and then ran
the PCR reaction on ABI prism?

Theoretically this should be work but I have no idea of the starting
RNA and the number of cycles of the assymetric PCR. Please feel free to
forward any of your thoughts about this. I will compile any personnel
answer and forward it on the newsgoup.



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