Sequencing Vectors containing CMV promoters

[Alexander Borowski]

I have some problems in sequencing clones inserted in vectors containing th=
e
CMV promoter on an ALFexpress (pCR3.1, pBK-CMV). My question is, if there i=
s
anyone who noticed the same problems with automated sequencing of CMV
containing vectors?
___________________________________________________________________________
Dipl. Biol.=20
Alexander Borowski
Immunologisches Labor=09=09=09=09      Tel.:+49 211/81-16198
Institut f=FCr H=E4mostaseologie und Transfusionsmedizin     =09      18577
Heinrich-Heine-Universit=E4t D=FCsseldorf=09=09      =09=09      14782
Moorenstr. 5, 40225 D=FCsseldorf, Germany=09=09      Fax: +49 211/81-16221
e-mail:=09alexander.borowski at uni-duesseldorf.de
___________________________________________________________________________