Resolution problems at 200-250 base pairs.

Andy Hopwood "106617,2551" at compuserve.com
Mon Jun 1 14:35:29 EST 1998


Attention all the users out there that have seen Resolution problems at
200-250 base pairs.
We have been seeing this phenomenon on and off for a while now, although
tracing the absolute cause has been evasive. Like other users, we have
felt that plate cleanliness is a critical part of the problem.
We are using a TBE based system with formamide to denature the samples. 
Has this problem been seen with a different buffer and/or when urea is
used to denature the samples?
Any help would be really welcome!!
Regards
Alex Connolly





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