HELP - ABI 310 low current/no signal problems
rrumpf at postbox.acs.ohio-state.edu
Wed Jun 3 14:53:00 EST 1998
Okay, I'm a newbie to running the 310. First run appeared okay,
although the signal didn't start appearing until more than an hour
into the run (so I only got about 300 bases of the control sequence).
All parameters were within the normal specs, except current flow -
it was a steady 2 microamps the whole time. Isn't this a bit low?
What might cause this?
Also: Took the same reaction that gave me the good signal in the
first run and repeated the run (same sample tube; I didn't aliquot
the reaction prior to this). On this run I got no signal whatsoever.
Is it possible I depleted the sample of, well, sample? :)
Any advice/comments are appreciated...
For reference, here's what I'm using:
250 ul syringe
dRhodamine sequencing kit
POP6 Seq (250ul) E module
More information about the Autoseq