Inverted repeats

David.Fruhling at cadus.com David.Fruhling at cadus.com
Fri Jun 12 14:01:01 EST 1998


I am having trouble sequencing through a region of inverted repeats.  I
have tried building new primers and raising the temperature of my primer
annealing and extension steps.  None have worked.  I still get 150 very
strong bases and then the sequence drops off dramatically.  Any hints,
chemicals I can add to the reactions, etc.

Thank you,
David S. Fruhling

david.fruhling at cadus.com
Bioinformatics
Cadus Pharmaceuticals




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