loading systems

Your Name PatronID at glaxowellcome.com
Wed Jun 17 21:36:26 EST 1998


Tom,

We have been working with these combs for several months now and really
like them a lot.  In fact, we're about to try the new 64-lane combs we ju=
st
got in today. =20

There were some early versions of the comb that had an adhesive with blue
fluorescent properties, but Genetic Biosystems fixed that and we haven't
seen problems since.

The biggest advantages I see are that the porous combs can be loaded on t=
he
bench-top (and thus the loading can be automated) up to 48 hours ahead of
time.  Also, there are no more problems with shark's tooth comb leakage!!=
!=20
(The sample volumes we load on the porous comb are 0.4ul so as to not all=
ow
the samples to wick up past the individual tabs and merge=85which would i=
n
essence, be the only possible equivalent of "leakage" for these combs.)  =
=20
Furthermore, there's no loading of odds and then evens and there's no
pre-run necessary.  Quite a lot of time savings. =20


The only other advice I can give is to make sure that there is very minim=
al
delay time from the point that the sample loading solution is added to th=
e
well area to the time run is pressed.  My understanding from Genetic
Biosystems is that leaving the solution in contact with the gel for
extended periods of time before running causes changes in the urea
concentration, resulting in compressed lanes at the end of the run (top o=
f
the gel image).  =20


Michele Godlevski


Laboratory Supervisor
DNA Sequencing Facility=20
Department of Molecular Genetics
Discovery Genetics Division
GlaxoWellcome, Inc.
483-3801 (office) 483-3744 (lab)
Venture 077

mam7746 at glaxowellcom.com

Thomas J. Stelick <tjs11 at cornell.edu> wrote in article
<6m4159$4cj at net.bio.net>...
> I was wondering if anyone is using the Genetic Biosystems porous comb
> loading system for the 377 and how it has worked for them.  I have seen
> data from that loading system that looks good and others that look bad.
> Has anyone got any tricks to making it work better?
>=20
> Tom Stelick
>=20
> BioResource Center
> Cornell University
> (607)254-4857
> tjs11 at cornell.edu
>=20
>=20
>=20




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