1998 ABRF DNA Sequencing Research Committee Study latest results

George S. Grills grills at aecom.yu.edu
Wed Nov 11 16:06:35 EST 1998

     The latest results from the 1998 ABRF DNA Sequencing Research Committee
Study are now posted at
The title of the study is “Analysis of the Effects of Different DNA Sequencing
Methods on Sequencing Quality, Creation of a Quality Control Resource, and
Assessment of the Current State of the Art.”  
     The study examined the results of sequencing a standard template and
included 242 sequencing results from 72 machines in 59 laboratories. The first
goal of this study was to analyze the effect of a wide range of commonly used
sequencing methods and instrumentation on the quality of sequencing results.
The effects of factors such as different machine types, dye chemistries,
reagent dilutions, and editing were examined.  The second goal was to create a
readily accessible web based resource of sequencing results.  This resource can
be used as a benchmark for sequencing, as a reference for self-evaluation, and
for making decisions concerning new technologies.  The third goal was to build
a current profile of DNA sequencing laboratories. 
     Preliminary results were presented in March 1998 at the ABRF annual
meeting and were posted on the web.  A comprehensive analysis of the data was
presented in September 1998 at the 10th International Genome and Sequence
Analysis Conference.  The details and an update of this latest analysis are now
posted on the ABRF web site. 
     Please note that the ABRF DNA Sequencing Research Committee will continue
the 1998 study of different conditions on a standard template in 1999.  This
1999 study will include analysis of results using the latest new technologies
and chemistries.  In addition, the DSRC is preparing challenging ‘problem’
templates that will be the focus of a new 1999 study.  The long term goal of
the new study will be to identify an array of templates that can be used as DNA
sequencing standards for evaluation and troubleshooting.  Details of how to
participate in the 1999 studies will be posted in December 1998.

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