Plasmid Sequencing Question

[Eric C. Buxton]

Has anyone ever had a problem with sequence where it starts out really
messy with multi-peaks,  and then the sequence cleans up to one
sequence, but with terrible resolution?  The template has worked before
with the primers used.  This is on a gel where other samples have ok
resolution, so it doesn't seem to be a gel problem..  Is the problem
with the amount of template used?  We are using BigDyes on a 377.
Thanks,
Eric Buxton