Plasmid Sequencing Question

Eric C. Buxton buxton at
Wed Nov 25 18:21:53 EST 1998

Has anyone ever had a problem with sequence where it starts out really
messy with multi-peaks,  and then the sequence cleans up to one
sequence, but with terrible resolution?  The template has worked before
with the primers used.  This is on a gel where other samples have ok
resolution, so it doesn't seem to be a gel problem..  Is the problem
with the amount of template used?  We are using BigDyes on a 377.
Eric Buxton

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