cDNA library sequencing

Wes Keys wesk at pi.csiro.au
Tue Sep 1 16:27:44 EST 1998


I am starting a large sequencing project of single pass sequencing from a
cDNA library. The library is cloned in the Bluescript vector stored in 384
microtiter plates. Does anybody have a protocol suitable for sequencing
large numbers of clones that could be automated on the Beckman Biomek work
station.
In particular I was wondering if you could PCR up the insert from the
clone, without the need to extract the plasmid via a plasmid prep. step,
then sequence the product.
Thanks for your help, Bryan.





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