gel extrusion 2

Harold G. Hills hhills at iastate.edu
Tue Sep 1 16:30:36 EST 1998


Hi,

I'm posting a bit of information in quotes that was sent to me.

"">When we had the extrusion problem, we got extrusion from a brand new set
>of plates from a company that swore that extrusion was a function of glass
>lot and that theirs were prescreened not to extrude.  The extrusion came
>about from soaking in something, don't remember details.  My glass expert
>was skeptical of the charge concept and I was able to cause extrusion by
>soaking overnight in diwater.  We usually Alconox new plates well to get
>rid of the film and then water wash from there except when trying to cure
>problems like extrusion.
>
>I still suspect there may be a missing factor like a particular lot of
>reagent or build up on plates that triggers it.  Long Ranger also hasn't
>extruded for us when other things were extruding.  RIght now we are just
>drying in the oven overnight (~30'C) and not baking, waiting to see what
>happens.  Long Ranger (current standard) still hasn't extruded but Rapid
>Ranger (we were testing it) has given repeated extrusion problems.""

Now, how many of you who are not have extrusion problems are using
LongRanger.  We use 29:1 acrylamide from Amresco because I could not get
consistant results from LongRanger doing 4x runs.  We do all 4X runs.  I
also could not get consistant results from PagePlus.  The person above was
using PagePlus and switched to LongRanger.

Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.




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