Sequencing 16S rRNA genes

A Johnson ajohnson at
Tue Sep 29 08:05:07 EST 1998

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Hello all,

     I have a user who has sent me pGEM templates containing 1.4kb inserts
of 16S rRNA genes. I've attempted to sequence them with both dRhodamine
terminators and BigDye terminators with 5% dmso, but no luck. Is anyone out
there sequencing 16S with success, and if so, how are you doing it?

A Johnson, Ph.D.
NCSU DNA Sequencing Facility
Tel: (919)-513-1007
FAX: (919)-515-7801
Email: ajohnson at

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