In response to the problems of loading 96 lane gels.
I visted a genome center where they were using the standard plates with a
96 lane 0.2 mm comb and loading much as you would with 64 lanes; that is a
multi-channel loader. No water loading, no lane markers.
We have just started to use the 96 lanes with a 0.2mm comb from the Gel
Company. It does not seem to be a big deal to load with the multi-channel
loader. We load our 80 lane gels one at a time.
Tracking is never going to be perfect because the gels are not perfect.
Yes, it takes a little time to verify the tracking.
Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist 515 294-9585
1184 Molecular Biology Building FAX 515 294-1597
Iowa State University hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF For info about facility.
