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DNA Seq 96 lanes

Harold G. Hills hhills at iastate.edu
Tue Aug 24 03:56:42 EST 1999


In response to the problems of loading 96 lane gels.

I visted a genome center where they were using the standard plates with a
96 lane 0.2 mm comb and loading much as you would with 64 lanes; that is a
multi-channel loader.  No water loading, no lane markers.

We have just started to use the 96 lanes with a 0.2mm comb from the Gel
Company.  It does not seem to be a big deal to load with the multi-channel
loader.  We  load our 80 lane gels one at a time.

Tracking is never going to be perfect because the gels are not perfect.
Yes, it takes a little time to verify the tracking.

Hal
Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.




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