Loading system for 96 lanes.

Stephen R. Lasky srlasky at u.washington.edu
Tue Aug 31 11:37:24 EST 1999


Louis Geller wrote:
> 
> Hi.
>     My lab wants to upgrade to 96 lanes for Sequencing and Genescan
> using an ABI-377-96.  We have the instrument and have been shown the
> protocol by Perkin-Elmer.
>     The problem is what type of loading system to use.  I would like to
> get some information about what multi-channel pipettes people are using,
> what they think of them, and what problems they have encountered with
> the various systems.
>      I would appreciate any help!
> 
>     Louis Geller


We use the Kloen pipetting system.  This works well.  It was a steep
learning curve and we went through a lot of needles in the first couple
of weeks, but once the techs learned how to use it, it became less
expensive than disposable tips. The nice part about the Kloen is that
the tips are small enough that you can load the wells from the bottom
up, which is the way PEbiosystems recommends.  It makes a difference in
the resolution of the bands.

Robbins has a new pipettor out that has flexible tips. That might be
interesting to try, but we aren't willing to buy one to try it.

Hope that helps

srlasky


-- 
Stephen R. Lasky, Ph.D.			#
University of Washington		#
Department of Molecular Biotechnology	#
srlasky at u.washington.edu		#
#########################################




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