Victor DeFilippis wrote:
>> Hello,
>> I have been using the Long Ranger Singel packs for running 36 cm gels on an
> ABI 377. They have proven to give fairly consistent results and are quite
> easy to use. However, lately we've had a lot of problems with them. Does
> anyone know of a competitive product made by another company? I'd like to
> sample something else before I invest more money in the Singels.
>> Thanks in advance.
>>vrdefili at uci.edu
Amresco has and EZ-squeeze gel product that is a premix like FMC
(actually easier and faster to use). So does Sigma. Both work well. We
were unable to detect any difference in quality of our seqeunces between
the different acrylamides.
It's really expensive to use the singel/premixed type of acrylamide.
You can get FMC and Amresco acrylamide concentrates in 40 and 50%'s and
then add your urea and deionize, filter and add TBE, TEMED and APS
yourself and save yourself a lot of money. You could also mix your own
acrylamide/bis from solid (we didn't find a great cost savings doing
this over the concentrates, so we don't do it). If you have one or a few
people making the mix, you can control quality just as well as they do
in the premixes and significantly cut your gel costs.
Hope that helps.
srlasky
--
Stephen R. Lasky, Ph.D. #
University of Washington #
Department of Molecular Biotechnology #
srlasky at u.washington.edu #
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