310 96well format
jtaylor.ebi at erols.com
Wed Dec 15 20:33:39 EST 1999
I have recently ramped up to processing up to 96 samples at a time on my
310. While I realize that I can not load the entire plate due to the speed
of the machine. I can process 48 at a time.
The problem I am having is that I run the cycle sequence reaction with the
BD kit in the 0.2 tubes using an Eppendorf Thermal Cycler. I then proceed
to the precipitation stage following the protocol supplied by ABI.
Next I spin the DNA using an Eppendorf centrifuge. I wonder if the DNA is
spinning down properly.
I was running 0.25x reactions with great results. Now, however, I get junk
sequence. Even at full concentration.
I use a quantitative ladder to estimate my plasmid concentrations, which
were prepared using the Quigen mini-prep system.
Any help at all would be greatly appreciated,
More information about the Autoseq