DNA sequence paired lanes

Harold G. Hills hhills at iastate.edu
Tue Jan 12 18:44:19 EST 1999


I have been having a problem with lanes that start out seperate but as the
gel runs, lanes will merge so that they will be overlapped at the end of
the gel.  I've tried various things but can't seem to get a consistant
solution to the problem.  The worse problem is that I seem to be the only
one that has the problem on a regular basis.  Happens at different
positions on the gel.  Was only happening on the left side, but now seems
to happen in the center.

I prerun for 2 minutes between loading.  Loading manually.

I'll post a couple pictures at our FTP location.  I'm not a computer whiz,
so I won't give you a URL.

Host: sequence.bb.iastate.edu

UserID:  ABRF

password: abrf

Look for a file that says paired lanes
Harold G. Hills, Ph.D. DNA Sequencing Specialist	515 294-9585
1184 Molecular Biology Building			    FAX 515 294-1597
Iowa State University                                   hhills at iastate.edu
Ames, IA 50011-3260
http://www.biotech.iastate.edu/Facilities/DSSF/ABRF   For ABRF 97 DNA
sequencing tutorial.
http://www.biotech.iastate.edu/Facilities/DSSF   For info about facility.




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