Can we use less BigDye terminator reagent mix?

[Adrian Jenkins]

Morning,

I/we routinely use half volume reactions with out any general problems.

Sometimes samples which have failed on 4 µl BigDye terminator reagent have
worked using 
8 µl BigDye.

Hope this helps

Adrian

Rocky Ho wrote:
> 
> I am now using 8 µl BigDye terminator reagent mix for 20 µl sequencing
> reaction. Is there any possible suggestion for reducing the use of the
> reagent?
> 
> - Rocky Ho

-- 


Enjoy....

Adrian

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        NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL

        Adrian Jenkins          Molecular Virology Group
				Division of Retrovirology               
                                e-mail     ajenkins at nibsc.ac.uk         
                                http://www.nibsc.ac.uk/      
          
	"No plan survives contact with the enemy"  von Moltke

	"To Errr  is human, but when the eraser wears out
	ahead of the pencil; you're overdoing it."
							  Anon

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