Use 4 µl Big Dye Terminator reagent , it works fine to sequence pcr product
of 500 bp
Grégory Raux
Laboratoire de génétique moléculaire
Faculté de médecine et de pharmacie
22 boulevard gambetta
76183 Rouen Cedex
Rocky Ho <RockyHo at cuhk.edu.hk> a écrit dans le message :
7lnv4e$h0t at net.bio.net...
> I am now using 8 µl BigDye terminator reagent mix for 20 µl sequencing
> reaction. Is there any possible suggestion for reducing the use of the
> reagent?
>> - Rocky Ho
>
