Can we use less BigDye terminator reagent mix?

Grégory Raux gregory.raux at wanadoo.fr
Mon Jul 5 17:15:41 EST 1999


Use 4 µl Big Dye Terminator reagent , it works fine to sequence pcr product
of 500 bp

Grégory Raux
Laboratoire de génétique moléculaire
Faculté de médecine et de pharmacie
22 boulevard gambetta
76183 Rouen Cedex


Rocky Ho <RockyHo at cuhk.edu.hk> a écrit dans le message :
7lnv4e$h0t at net.bio.net...
> I am now using 8 µl BigDye terminator reagent mix for 20 µl sequencing
> reaction. Is there any possible suggestion for reducing the use of the
> reagent?
>
> - Rocky Ho
>





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