Rocky Ho (RockyHo at cuhk.edu.hk) wrote:
: I am now using 8 µl BigDye terminator reagent mix for 20 µl sequencing
: reaction. Is there any possible suggestion for reducing the use of the
: reagent?
We routinely do 1/8 BDTerm reactions. 1ul BDT mix + 2ul 5x buffer
(listed earlier) + DNA + 20pmol primer, and water for a 10ul rxn. This
works great for our M13 templates and PCR reactions. We load 1/2 of this
on our 96-lane gels and 1/6 to 1/4 of this on our 3700.
---
Paul Shinn
Sequencing Coordinator ,___o
pshinn at neomorph.bio.upenn.edu _-\_<,
Arabidopsis thaliana Genome Center (*)/'(*)
http://genome.bio.upenn.edu/ATGCUP.html
(215) 573-7256
