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Sequencing E.coli Chromosome

Steve Qi sqi at ferring-research.co.uk
Mon Jul 12 18:32:02 EST 1999


Dear sequencing news group,

I need to sequence part of E.coli gene using its chromosomal DNA as the
template.

Anybody has any experience in it using ABI big-dye terminator chemistry?

Or other machines, such as Li-Cor automated sequenecer?

Any suggestion will be very useful to me.


Thanks.


Dr. Yan Li

Department of Biochemistry
Universith of Southampton
Bassett Crenscent East
Southampton, SO16 7PX
Tel. 01703 594324
Email yl at soton.ac.uk





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