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PE 3700 Recommendations?

Bernd Weisshaar weisshaa at mpiz-koeln.mpg.de
Sun Jul 18 16:23:36 EST 1999


Dear Bob,

we use water to replace the formamid and it works much better.

Other question: which 3700-specific modification do you refer to for 
the "G50 Millipore method"?

Best regards, Bernd


>Dear Sequencers (and Genotypers),
>
>A while back I asked for recommendations on the best way to purify 
>BDT sequencing reactions for the PE 3700.  Following are the 
>recommendations I received:
>
>For 96 well plates
>Modified G50 Millipore method
>
>For 384 well plates
>Array-It from Telechem
>
>Now I have one more question for more experienced PE 3700 users. 
>How do you purify your formamide?  There are two protocols from 
>PE-ABI for purifying formamide, one using a mixed bed column method 
>and one by the standard stirring/filtration with mixed bed resin. 
>From our results with the 3700 it looks like the purity of the 
>formamide is critical for good results.  We can get over 650 base 
>pairs of good sequence with the first run using HD Formamide from PE 
>but later runs (reinjecting the same samples) show significant 
>degradation in the length and quality of the sequences.  Any tips 
>would be greatly appreciated.
>
>Best Regards,
>
>Bob
>
>
>_______________________________________________________________________
>
>Robert B. Chadwick
>Director, Genotyping/Sequencing Unit
>Division of Human Cancer Genetics
>Ohio State University
>420 West 12th Avenue
>Columbus, OH   43210
>
>phone  (614) 688-4783
>FAX      (614) 688-4761
>e-mail   chadwick-1 at medctr.osu.edu
>web address  http://gsu.med.ohio-state.edu/index.htm
>__________________________________________________________

_____________________________________________________________________

PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-Linné-Weg 10
50829 Koeln
Germany

Tel. office: +49-221-5062 590   Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
mailto:weisshaa at mpiz-koeln.mpg.de
WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html
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