we use water to replace the formamid and it works much better.
Other question: which 3700-specific modification do you refer to for
the "G50 Millipore method"?
Best regards, Bernd
>Dear Sequencers (and Genotypers),
>>A while back I asked for recommendations on the best way to purify
>BDT sequencing reactions for the PE 3700. Following are the
>recommendations I received:
>>For 96 well plates
>Modified G50 Millipore method
>>For 384 well plates
>Array-It from Telechem
>>Now I have one more question for more experienced PE 3700 users.
>How do you purify your formamide? There are two protocols from
>PE-ABI for purifying formamide, one using a mixed bed column method
>and one by the standard stirring/filtration with mixed bed resin.
>From our results with the 3700 it looks like the purity of the
>formamide is critical for good results. We can get over 650 base
>pairs of good sequence with the first run using HD Formamide from PE
>but later runs (reinjecting the same samples) show significant
>degradation in the length and quality of the sequences. Any tips
>would be greatly appreciated.
>>Robert B. Chadwick
>Director, Genotyping/Sequencing Unit
>Division of Human Cancer Genetics
>Ohio State University
>420 West 12th Avenue
>Columbus, OH 43210
>>phone (614) 688-4783
>FAX (614) 688-4761
>e-mail chadwick-1 at medctr.osu.edu>web address http://gsu.med.ohio-state.edu/index.htm>__________________________________________________________
PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Tel. office: +49-221-5062 590 Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
mailto:weisshaa at mpiz-koeln.mpg.de