TTE Buffer

Skip Vaught, Brian Coullahan, Mark Schwartz, and Felisa Blackmer Skip Vaught, Brian Coullahan, Mark Schwartz, and Felisa Blackmer
Sat Jun 12 14:30:55 EST 1999


Dear DNA Sequencing Friends,

I'm interested in the reputed longer read protocols using TTE buffer
instead of TBE. Anyone out there using TTE?  What's your verdict? When I
checked the cost of TAPS (the substitute for Boric Acid), I almost fell off
my chair. ABI's recipe for 1 liter of 10X TTE Buffer:

0.5M   Tris-base   60.55 grams
0.5M   TAPS-acid   121.65 grams (Sigma part number T9659)
10mM   EDTA        3.72 grams

The cost (from the Sigma Catalog) of 1 liter's worth of TAPS is
approximately a whopping $40! ( 1kg - $355.50)

Is the stuff worth it?  Are there less expensive sources?

Thanks for your help.

Skip Vaught

DNA Sequencing Service
University of Arizona


THE DNA SEQUENCING SERVICE
UNIVERSITY OF ARIZONA

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Skip Vaught-     jev at u.arizona.edu
Brian Coullahan- coullaha at u.arizona.edu
Mark Schwartz-   schwartm at u.arizona.edu
Felisa Blackmer- blackmer at u.arizona.edu





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