3700 top-heavy data

Amy Avery AAvery at pavonis.com
Wed Jun 16 16:50:17 EST 1999


We obtain ~350 good bases from our new 3700 sequencers, but would like
to get more.  The signal is high in the beginning and then falls off
early.  The raw data view looks like a ski slope, with the signal
decreasing after about 200 bases, even on the control sample.  We have
ruled out too much template and suspect the injection favors smaller
fragments.  Any suggestions for changing run or reactions conditions?

Thanks in advance,
Amy

Please reply to aavery at pavonis.com

Also, anyone know of a cheap/user-friendly Primer Design program?  How
about recommendations for microarrayers for cDNA library screening and
gene expression?  




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