Fluorescense quenching

Mark E. Berres markb at ravel.zoology.wisc.edu
Tue Mar 16 18:48:36 EST 1999


Does anyone have any knowledge concerning the maximum
amount of fluorescense an ABI 377 can detect and still
maintain a linear relationship between the mass of
fluorophore labelled DNA and fluorescense intensity?

In my experience, it seems to be somewhere around 4100
(rfu) but this is only empirical.

			Thanks!

				Mark
-- 


Mark E. Berres				4-360 Genetics
Department of Genetics  		445 Henry Mall
Department of Zoology			Madison, WI. 53706-1313	
University of Wisconsin at Madison	Ph: (608)-262-5578




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