Fluorescense quenching

Mark E. Berres markb at ravel.zoology.wisc.edu
Tue Mar 16 18:48:36 EST 1999

Does anyone have any knowledge concerning the maximum
amount of fluorescense an ABI 377 can detect and still
maintain a linear relationship between the mass of
fluorophore labelled DNA and fluorescense intensity?

In my experience, it seems to be somewhere around 4100
(rfu) but this is only empirical.



Mark E. Berres				4-360 Genetics
Department of Genetics  		445 Henry Mall
Department of Zoology			Madison, WI. 53706-1313	
University of Wisconsin at Madison	Ph: (608)-262-5578

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