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Sequencing without PCR purification

Joao Alexandrino jmalexan at mail.icav.up.pt
Tue Mar 16 18:48:18 EST 1999


I would like to know if someone has obtained good results with ABI 
BigDye sequencing without purifying PCR products (from excess 
primers and dNTPs)?
Thank you, Joao

Joao Alexandrino
Centro Estudos Ciencia Animal-ICETA
Unidade de Genetica Animal e Conservacao
Campus Agrario de Vairao
R. Monte - Crasto - Vairao
4480 Vila do Conde, Portugal
tel. 351 52 662399  fax. 351 52 661780
email: jmalexan at mail.icav.up.pt 

Joao Alexandrino
Dept. Zoologia e Antropologia
Faculdade de Ciencias 
Universidade do Porto
email: jmalexan at fc.up.pt

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