Accuracy of PCR product seq.

Anders Holmberg cato at biochem.kth.se
Mon Mar 29 12:57:32 EST 1999


At 11:12 1999-03-26 , you wrote:
>In article <7delfn$141 at net.bio.net>, radomski <radomski at unixg.ubc.ca>
>writes
>>Has anyone come across an article that deals with the overall accuracy of 
>>sequencing PCR products? I just replied that the error is 
>>probably the same as the error rate of the Taq used in the PCR.
>

The error will most probably not show up in a "normal" sequencing run!

Worst case scenario:
You have 1 (one) DNA copy in your PCR...
An error is introduced in the first run....
You end up with a 1:4 ratio for that base...
Normally you have problems to distinguish
heterocygozity below 1:10

/Anders
***********************************************
Anders Holmberg
Royal Institute of Technology (KTH)
Dep of Biochemistry
100 44 Stockholm, Sweden

Phone : +46 8 790 82 87
Fax         : +46 8 24 54 52
Email : cato at biochem.kth.se
Web         : www.biochem.kth.se
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