I really don't profess to be any sort of genotyping expert, but I know some
polymerases, if RXN conditions aren't exactly right tend to throw an A onto the
end of PCR products if there isn't already one. It's my understanding in fact
that some groups tend to optimize thier RXNs so the only n+1 product appears,
thus giving consistant single peaked results. Again, I'm not the world's
expert, but play with the idea. Good luck and let me know what you figure out
if you have time.
Alison Brown wrote:
> I've been asked a question which I can't answer and I wondered if any
> genotypers out there could help out. We are using the LMSv2 marker set from
> ABI to do high through-put genotyping. Several groups have found primers
> which give alleles 1 bp apart - the question is how to people deal with them
> - are they ignored, or put into normal bins - if so which one, the one
> before or the one after.
Institute for Biosciences, Bioinformatics, and Biotechnology
George Mason University, Manassas VA
Phone: (703) 993-8463 Fax: (703) 993-8460
paul at ib3.gmu.edu www.ib3.gmu.edu
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