Low temperature optimized 3700 basecaller

Phillip San Miguel pmiguel at purdue.edu
Wed Nov 3 04:38:45 EST 1999


    Has anyone tried the low-temperature-optimized
basecaller etc. for the 3700 on the PE web site[1]? I just
tried it out on some reads generated the day before
yesterday. I mainly used Robert Chadwick's protocol for
running [2] with the injection time/voltage change suggested
recently by Stephen Lasky [3].
    I was hoping to see improvements in my phred q>20 scores
but I saw the opposite:

number of
phred q>20
bases              old         new

0-49        4      4
50-99       1      1
100-149     1      2
150-199     2      1
200-249     4      4
250-299     0      0
300-349     0      1
350-399     4      2
400-449     3      4
450-499     1      1
500-549     6      6
550-599    11     20
600-649    32     45
650-700    26      5
701-750     1      0

Any comments?

Phillip San Miguel

[1]  http://www.pebio.com/ab/techsupp/swpps/3700sw.html
[2]
http://www.bio.net/hypermail/AUTOMATED-SEQUENCING/9908/0024.html

[3]
http://www.bio.net/hypermail/AUTOMATED-SEQUENCING/9910/0060.html







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