multiple basecalling errors
Darek.Kedra at cmm.ki.se
Tue Oct 12 12:22:45 EST 1999
I am an ABI 377 user. We recently switched from Perkin Elmer BigDyes kit
to Amersham's DYEnamic ET terminators.
Surprisingly after few good runs we got a series of gels with multiple
basecalling errors. Instead of single As, Cs Gs and Ts, we are getting
several of them (AAAs and so on). I have remade the matrix file and
copied the original "DYEnamic ET Term (US80872)" mobility file, but to
Any comments and suggestions will be very much appreciated.
Darek Kedra, M.D. e-mail: Darek.Kedra at cmm.ki.se
Center of Molecular Medicine (CMM), Karolinska Hospital,
Building L-8.00, S-171 76 Stockholm, Sweden
phone: +46-8-5177-3922 fax: 46-8-517 73909
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