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Rui Mang r.mang at amc.uva.nl
Thu Oct 14 14:03:33 EST 1999


Dear all,

I am working with 377 for lambda DNA sequencing.  I have lots of question
about my sequencing work, for example:

1. How I can make 48 cm running to read 700 to 900 base, (now I can read
only 400-500 base)?
2. I am using the Wizard Lambda DNA purification system from Promega to
prepare my template for Big-Dye Terminator reaction and the sequencing
results are not very stable, sometime is reasonable, and sometime is very
bad. Does anybody know how to deal with this sequencing work?

Thank you for your help in advance.

Rui Mang
Department of Human Retrovirology
AMC, University of Amsterdam
Meibergdreef 15
1105 AZ, Amsterdam
The Netherlands
Tel: 31-20-5664522
Fax: 31-20-6916531
Email: r.mang at amc.uva.nl




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