cosmids

Dave Fruhling Fruhlid at war.wyeth.com
Tue Oct 19 16:14:09 EST 1999


I have been given the uneviable task of having to sequence a cosmid.  The cosmid is pWE15 from stratagen and has a 3kb insert. having never sequenced any larger 
than a big plasmid (10-11kb including insert).  I was wondering if anyone has any special hints or stratagies for sequencing a cosmid.  Do I need to add Mg or 
change cycling parameters, longer/shorter annealing times. 
Any help is greatly appreciated 
David S. Fruhling 
Wyeth Ayerst Research 
fruhlid at war.wyeth.com 




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