Dear Barbara, dear Christian, dear DNAsequ'ers
the 1000bp protocol for 377 was developed by the German PE support
stuff and tested in a number of German labs. For us, it worked quite
nicely since quite some time, so I mentioned it in an Email to the
group assuming that PE has already distributed it. After a number of
replys asking for the protocol, I tryed to find the PE web page which
has the protocol we got, but I failed to find that page.
After that, I leaned that PE Foster City is still testing the
protocol. They are planning to prepare a User Bulletin which includes
an official "1000bp protocol" (beginning of 2000). To get the
protocol now, you should contact your local PE support people. They
should be able to give you the details. The reason for this way of
action is that the support people want to get things set up in the
best way. Implementation of the protocol is not too difficult, but
also in our case some tips and tricks from the support were helpful
to get it going.
The trick to increase data collection time is simply to restart the
gel run at the time the first signals are detected from the 48-wtr
gel. This way, you discard all the "black" data collected during the
time the smallest fragments need to reach the detection area.
Hope this helps. You may contact me directly if not.
All the best, Bernd
>>This is a copy and paste of Christian Vasseur's message directed to you,
>since it says the same words I would write to you:
>>could you provide us the PE "1000bp protocol" or an URL where we could
>I am unaware of it but very interested in it. And also what are you
>thinking about when you say "and you have to play tricks to increase
>data collection time."
>>Thank you for your help.
> Barbara Simionati
> GRUP Sequencing Core
> CRIBI Biotechnology Centre
> and Department of Biology
> University of Padua, Italy
PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Tel. office: +49-221-5062 590 Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
mailto:weisshaa at mpiz-koeln.mpg.de