Hi all,
Try disolving your samples in a little more water and you'll
be supprised how good the results for the 3rd and 4th plates are without
the addition of the 'fang'. Not to anyone's supprise, the water in
the 96 well plates evaporates at a rate of ~1.5 ul/hour here in Oklahoma
in the summer. Thus, we add an additional 10 ul of water to each well
in the second plate, 20 ul more to the 3rd plate and 30 ul more to the
4th plate. The amount you add may need to be adjusted depending on
the relative humidity in your location. Guess if we lived in Arizona
we'd have to add more water and in Miami, add less. So do the expt and
the math and add additional water as need so that the samples don't dry
out. Water is alot cheeper than having to deal with a, IMHO, unnecessary
additional upgrade.
Oh yes, while I'm thinking of it. The waste bottle had a gazillion
pieces of tubing that are passed through the screw cap. We've wrapped
them with a couple of rubber bands (you know me, bailing wire and duck
tape and maybe a few rubber bands and a swiss army knife and we can fix
anything) to keep the techs from inadvertantly pinching off a tube or
two by not getting them all inserted into the container before screwing
down the top..... sigh.
Cheers and happy sequencing,
--bruce
*************************************************************************
Bruce A. Roe, Ph.D George Lynn Cross Research Professor
Department of Chemistry and Biochemistry
University of Oklahoma, Norman, OK 73019-0370, U.S.A.
Phone: (405) 325-4912 or 7610; FAX: (405) 325-7762; e-mail: broe at ou.edu
********************** http://www.genome.ou.edu/ ************************
