we are running long ranger gels in our lab at 5.25% with TBE, also
degassing for 10 minutes, following the protocol completely, but our
resolution lately for the last ten days has not been good at all. lanes
are smeary, fading. we are also storing our premixes in the freezer and
thawing it before we use it. also the thawed acrylamide is in the
refrigeratot for a couple of days before we get to use it. we are also
thawing the acrylamide right when we get it and aliquot it out. I am
wondering if repeated thawing and freezing ( twice) causing a problem
with our gels. Also does anyone know how long we can use the loading dye
that is kept in the refrigerator, is there a break down time frame for
dye and for acrylamide?
any info will be very much appreciated.