sequencing BAC clone

Chun-yu Liu cliu at yoda.bsd.uchicago.edu
Wed Oct 27 04:27:44 EST 1999


Hi, all
I am trying to sequence BAC insert, but failed.
Right now, I am using the DNA prepared by Autogen 740 automated DNA
preparation device.
1- 4 ug DNA was tried according to OD260. I suspect there is something
left in the DNA after the Autogen740.
I've tried to warm the DNA at 60C for 20min to remove possible ethanol,
but no improvement yet.
Can somebody give me a hand?
Thanks a lot!
-- Chun-yu Liu
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R022, BSLC
924 E. 57th st.
Dept. of Psychiatry,   Univ. of Chicago
Chicago, IL60637
Tel: 773-834-3604 (O)  Fax: 773-834-2970
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