Problems with gel

Chun-yu Liu cliu at yoda.bsd.uchicago.edu
Wed Oct 27 12:07:30 EST 1999


We keep premixes in refrigerator for more than one month, it's still OK.
We never have any problem with loading dye while it's kept in refrigerator
for more than half an year.

kris wrote:

> Hi everybody,
>
> we are running long ranger gels in our lab at 5.25% with TBE, also
> degassing for 10 minutes, following the protocol completely, but our
> resolution lately for the last ten days has not been good at all. lanes
> are smeary, fading. we are also storing our premixes in the freezer and
> thawing it before we use it. also the thawed acrylamide is in the
> refrigeratot for a couple of days before we get to use it. we are also
> thawing the acrylamide right when we get it and aliquot it out. I am
> wondering if repeated thawing and freezing ( twice) causing a problem
> with our gels. Also does anyone know how long we can use the loading dye
> that is kept in the refrigerator, is there a break down time frame for
> dye and for acrylamide?
>
> any info will be very much appreciated.
>
> kris

-- Chun-yu Liu
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