Bruce, wanted to congratulate you on Chr.22 and getting a grant to do
the syntenic regions in mouse. That's great and you deserve to be doing
it.
Bruce Roe wrote:
>> Hi all,
> Try disolving your samples in a little more water and you'll
> be supprised how good the results for the 3rd and 4th plates are without
> the addition of the 'fang'. Not to anyone's supprise, the water in
> the 96 well plates evaporates at a rate of ~1.5 ul/hour here in Oklahoma
> in the summer. Thus, we add an additional 10 ul of water to each well
> in the second plate, 20 ul more to the 3rd plate and 30 ul more to the
> 4th plate. The amount you add may need to be adjusted depending on
> the relative humidity in your location. Guess if we lived in Arizona
> we'd have to add more water and in Miami, add less. So do the expt and
> the math and add additional water as need so that the samples don't dry
> out. Water is alot cheeper than having to deal with a, IMHO, unnecessary
> additional upgrade.
On the FANG. We were using it quite successfully but a ran into a
couple of problems with the robot arm not being able to find itself, a
sheared pin on one drive, and apparently it is really hard on the z-axis
drives, so we took it off and are using the increased solvent solution
again. We find that 15 lambda's additional solvent (20,35,50,65 ul)
works here in Seattle. Which is suprising considering that it rains all
the time here. Mayber our HVAC system takes out more water than they do
at OU. Obviously, the amount of solution you suspend in depends upon
your DNA prep etc. I just mentioning this in case someone is keeping
track of all these solutions.
srlasky
--
Stephen R. Lasky, Ph.D. #
University of Washington #
Department of Molecular Biotechnology #
HSB K324 box 357730 #
Seattle, WA, 98195 USA #
email: srlasky at u.washington.edu #
Phone: 206-616-5865 #
Fax: 206-685-7301 #
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