Paul Shinn wrote:
> OK, one more question about using water to resuspend samples. I've
> found when using only water, the initial peaks are VERY strong and then
> the signal quickly fades out. I started with the 50:50 of water and
> formamide/EDTA but the samples degrade by plate 4, however, the
> peaks were more even. For those of you running with water, is it just
> water or have you doped it with a touch of formamide or EDTA?
> Paul Shinn
> Sequencing Coordinator ,___o
>pshinn at neomorph.bio.upenn.edu _-\_<,
> Arabidopsis thaliana Genome Center (*)/'(*)
>http://genome.bio.upenn.edu/ATGCUP.html> (215) 573-7256
To resuspend our samples, we use 0,3 mM of EDTA (no formamide). We
have good results with this method. But maybe, there is a better method!