Major Gel Problems!

Andrew Louka andrew.louka at biotek.uio.no
Tue Apr 4 07:37:28 EST 2000


Hi Sonia,

I don't have the answer to your problem, but I notice that two people 
have pointed to the cleanliness of your plates.  You probably remember 
this newsgroup has been polled with impressive participation, about 
the problem of gel extrusion.  Many variations of acid or other washes 
were suggested as solutions, and this works for many people.  I would 
point out that for many people, their chosen protocol was sufficient - 
it worked for them.  They didn't work for us, and most labs had tried 
variations that had failed too.

It was suggested that we bake our plates at 70-80 degrees for 2 hours 
(or wash them in a dishwasher with a high temperature drying cycle).  
This is the only treatment that has succeeded in our lab (377XL), and 
is now the labs undisputed first treatment in the light of any gel 
problems that may be attributed to the plates.  The method has worked 
in our lab, every single time.  We have tried an acid wash, out of 
curiosity, twice, but these still fail in out lab.  Since you have 
tried an acid wash, I suggest you try baking your plates.  It may not 
work, but it's worth a try!

Good luck!

Andrew

>>>>>>>>>>>>>>>>>> Original Message <<<<<<<<<<<<<<<<<<

On 19/03/00, 04:18:14, Sonia Lottinville <Slotti1 at uic.edu> wrote 
regarding Major Gel Problems!:


> Hi,
> I have to  two ABI 377's and have been having a problem with 
consistent
> data. I recently switched to Amersco's 5.25% Gene-page and am also 
using
> their TBE packets for our 48cm XL runs. Recently, my gels appear to be
> splitting up the middle (blank in the middle with samples showing up 
on
> the edges)  and pushing the samples out of the reading range so I 
cannot
> track the gel accurately. The samples appear to be fine (the ones that
> show up). I've talked to Perkin-Elmers tech support and have perfomed 
an
> acid wash on the plates. I've also used the Seque-Strip from Sigma and
> am still having problems. I was wondering if anyone else has seen this
> sort problems on their gels before?
> Thanks in advance!
> Sonia

> ---








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