Reconstitution of reactions for the 3700

[Phillip San Miguel]

Michelle wrote:

> We are just starting to optimize our 3700 and I was wondering what
> people were using to reconstitute their reactions.  I've been using 50%
> Hi-Di/water.
>
> Thanks.
>
> ---

    We use ddH2O. About 1/5th of  the total reaction volume is placed in the well of
a 384-well plate in a volume of 15 ul.

Phillip San Miguel
Purdue Genomics Core Facility


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