we are planning to sceen for mutations in a chemical mutagenised
population of barley... i would like to know if anyone has used a
377, megabase, or 3700 for the detection of mutations using
mismatch cleavage enzymes??? this technique was outlined in
"nucleic acids research, 1998, vol 26, no 20 4597-4602"...
what is the maximum pool size??? do's and don'ts??? any particular
dyes to use??? multiplexing???
any feedback would be greatly appreciated...