Mutation Detection using fluorescent primers

David Caldwell dcaldw at
Fri Dec 8 09:47:57 EST 2000

hello all,

we are planning to sceen for mutations in a chemical mutagenised 
population of barley... i would like to know if anyone has used a 
377, megabase, or 3700 for the detection of mutations using 
mismatch cleavage enzymes??? this technique was outlined in 
"nucleic acids research, 1998, vol 26, no 20  4597-4602"... 

what is the maximum pool size??? do's and don'ts??? any particular 
dyes to use??? multiplexing???

any feedback would be greatly appreciated...

david caldwell


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