Mutation Detection using fluorescent primers
dcaldw at scri.sari.ac.uk
Fri Dec 8 09:47:57 EST 2000
we are planning to sceen for mutations in a chemical mutagenised
population of barley... i would like to know if anyone has used a
377, megabase, or 3700 for the detection of mutations using
mismatch cleavage enzymes??? this technique was outlined in
"nucleic acids research, 1998, vol 26, no 20 4597-4602"...
what is the maximum pool size??? do's and don'ts??? any particular
dyes to use??? multiplexing???
any feedback would be greatly appreciated...
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