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'Half BD' big dye diluent

Joyce Bennett jbennett at seprl.usda.gov
Mon Dec 18 09:21:22 EST 2000

We have an ABI 3700. I tested the halfBD diluent using BigDye terminator
version 2 reaction mix and pgem with m13 forward primer.  In my hands, using
3 ul of reaction mix and 3 ul of halfBD resulted in over 600 bp reads. Using
2 ul of each decreased the read length to 300 bp. I would suggest  getting a
free sample and trying it in your lab. 

Joyce Bennett

Microbiology Lab Tech
Southeast Poultry Research Laboratory
934 College Station Road
Athens, Georgia 30605
jbennett at seprl.usda.gov


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