Licor Sequencing

kerstin.hoef-emden at uni-koeln.de kerstin.hoef-emden at uni-koeln.de
Thu Feb 24 06:11:03 EST 2000


Hello,

Curtis Clevinger <clevincc at jmu.edu> wrote:
> I am currently trying to sequence PCR products using custom primers on a
> Licor 4000 system.  I have been follwing Licor protocols and the
> protocol found in the EXCEL II kit for licor sequencers.  I am not
> obtaining good sequences (ie lots of background). 

How do you purify the PCR products? Background may be caused by impure
template.


Regards,

Kerstin Hoef-Emden


-- 

	kerstin.hoef-emden at uni-koeln.de				 






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