96-well PCR purification

Alexandre Bolotine bolotine at biotec.jouy.inra.fr
Tue Jan 18 10:49:08 EST 2000


Bonjour,

After exoI(1-5 u)  + Shrimph phosphatase(1-5 u) treatment following enzyme
inactivation heating 65C(10') or 94C(5') your PCR samples are ready for
sequencing.

Alexandre Bolotine

Chun-yu Liu wrote:

> Dear All
> Is there any protocol to substitute Qiagen kit to purify PCR products on
> 96-well plate for preparing sequencing templates?
> Thanks!
> -- Chun-yu Liu
> **************************************
> R022, BSLC
> 924 E. 57th st.
> Dept. of Psychiatry,   Univ. of Chicago
> Chicago, IL60637
> Tel: 773-834-3604 (O)  Fax: 773-834-2970
> **************************************
>
> ---






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